Recombinant Protein Expression and Purification Services
- Recombinant protein expression using expression systems such as E.coli, Pichia pastoris, Chinese Hamster Ovary (CHO) and Baby Hamster Kidney (BHK) cell lines
- Protein refolding and characterisation
- Screening and optimizing expression conditions
- E. coli pellet production
- Inclusion body isolation
- Solubilization and refolding
- Endotoxin removal
- Affinity-tag purification: His-, Flag-, HA-tag protein
- Heparin affinity: Protein A/G affinity column, Con-A sepharose
- Ion-exchange Chromatography
- Immobilized Metal Affinity Chromatography
- Western-blotting
- SDS-PAGE