Animal Cell Culture
- Good cell culture and laboratory practices
- Cell culture media formulations and preparations
- Cell culture techniques: Revival of frozen cells, subculture of monolayer and suspension cells,
cell counting and cryopreservation - Cell viability and proliferation assays
- Cell culture contaminants identification and control
- Isolation and culture of cells from primary tumours and human vessels (based on availability)
- Detection of cell death mechanism (apoptosis/necrosis)
- Cell cycle analysis
- Immunostaining techniques using fluorescent microscopy and flow cytometry