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  Animal Cell Culture

Animal Cell Culture
  • Good cell culture and laboratory practices
  • Cell culture media formulations and preparations
  • Cell culture techniques: Revival of frozen cells, subculture of monolayer and suspension cells,
    cell counting and cryopreservation
  • Cell viability and proliferation assays
  • Cell culture contaminants identification and control
  • Isolation and culture of cells from primary tumours and human vessels (based on availability)
  • Detection of cell death mechanism (apoptosis/necrosis)
  • Cell cycle analysis
  • Immunostaining techniques using fluorescent microscopy and flow cytometry
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