Molecular Biology Services

We offer molecular services for isolates of bacterial, mammalian, insect and plant cells and our service portfolio includes –

• Isolation of DNA, RNA and protein
• Qualitative/quantitative analysis of DNA,RNA– by spectroscopy and agarose gel electrophoresis
• Protein analysis
  • SDS-PAGE
  • Western blot
  • Bradford’s assay
  • Bicinchoninic acid (BCA) assay
• DNA fragmentation studies
• Restriction endonuclease digestion and fragment mapping
• PCR primer design
• Vector construction
• DNA transformation – Preparation and transformation of competent cells
• Molecular cloning
  • Amplification of DNA by PCR and cloning into vector of choice
  • Amplification of cDNA by RT-qPCR and cloning into vector of choice
• Genotyping services – Analysis of genetic markers by PCR and/or RT-qPCR as applicable
  • Single-nucleotide polymorphism (SNP)
  • Microsatellites
  • Copy number variation (CNV)
  • Variable region genes
  • Homologous genes
  • Novel genes
  • 16S/18S ribosomal DNA
  • AFLP, RAPD, Cleaved Amplified Polymorphic Sequences (CAPS)/PCR-RFLP, Genotyping by sequencing (GBS)
• RT-qPCR based gene expression profiling
  • mRNA extraction from different sample types (cultured cells, whole blood, tissues)
  • Quantification of the extracted mRNA
• miRNA expression analysis – by RT-qPCR
• Protein expression and purification services (from bacterial, insect and mammalian cells) – by affinity chromatography
• Mutation analysis– by PCR, RT-qPCR and MLPA (Multiplex ligation-dependent probe amplification)
• Antibody purification services
  • Purification methods – Ammonium sulphate precipitation and chromatography (affinity, size-exclusion and ion-exchange chromatography)
  • Quality analysis– by SDS-PAGE, western blot and ELISA
• Mycoplasma detection–by PCR and RT-qPCR
• Detection of viral contamination– by PCR and RT-qPCR
• Blood coagulation factor analysis– by fibrometer