Workshops
Short-term custom-based workshops with specialised courses are offered in basic and advanced technologies, that include -
- Basic cell culture workshop
- Molecular biology workshop
- RT-qPCR workshop–mRNA isolation and quantification, primer design, cDNA synthesis, gene expression analysis
- Flow cytometry workshops – cell surface immunostaining and cell sorting techniques
- Fluorescent microscopy workshops– immunofluorescence techniques
GENELON PRIMER
Duration: 10 Days
- EXTRACTION OF DNA / PLASMID DNA FROM CULTURED CELLS OR PROKARYOTIC CELLS.
- AGAROSE GEL ELECTROPHORESIS AND QUANTIFICATION OF DNA.
- PREPARATION OF COMPETENT CELLS BACTERIAL TRANSFORMATION - HEAT SHOCK METHOD.
- PREPARATIONS OF X-GAL/IPTG LB AGAR PLATES FOR BLUE/WHITE COLONY SCREENING.
- ESTIMATION OF PROTEIN CONCENTRATION USING SPECTROPHOTOMETER.
- SUBCULTURING & CRYOPRESERVATION OF MAMMALIAN CELLS.
- PRINCIPLE OF CELL VIABILTY ASSAY(TRYPAN BLUE).
- PRINCIPLE OF REAL TIME PCR.
- IMPORTANCE OF FLOWCYTOMETRY.
- IMPORTANCE OF CYTOSTAINING (KARYOTYPE, CYTOTOXCITY AND IMMUNOCYTOCHEMISTRY) IN MAMMALIAN CELL CULTURE.
* Terms and Conditions Apply
GENELON PHORESIS
Duration: 15 Days
- RESTRICTION DIGESTION OF DNA AND ITS ANALYSIS.
- POLYMERASE CHAIN REACTION AND RESULT ANALYSIS.
- CLONING AND RECOMBINANT DNA TECHNOLOGY(RDT).
- PROTEIN ISOLATION (CONVENTIONAL METHOD), DIALYSIS.
- PROTEIN ESTIMATION (PAGE AND SPECTROSCOPY).
- SAMPLE ANALYSIS IN FIBROMETER(PT AND APTT).
- BASIC OF MAMMALIAN CELL CULTURE (THAWING, SUB CULTURING AND CRYOPRESERVATION).
- CELL VIABILITY ASSAY(TRYPAN BLUE).
- RNA ISOLATION AND QUANTIFICATION FROM MAMMALIAN CELL.
- AMPLIFIACTION OF SAMPLE IN REAL TIME PCR AND RESULT ANALYSIS.
- BASICS OF CYTOSTAINING (KARYOTYPE) AND PRINCIPLE OF CYTOTOXCITY & IMMUNOCYTOCHEMISTRY.
- PRINCIPLE OF FLOW CYTOMETER.
* Terms and Conditions Apply
GENELON QUANTA
Duration: 30 Days
- PCR CLONING AND RECOMBINANT DNA TECHNOLOGY(RDT).
- ANALYSIS AND EXTRACTION OF DNA FROM AGAROSE GEL ELECTROPHORESIS.
- EXTRACTION OF PROTEIN (CONVENTIONAL METHOD) AND PURIFICATION OF PROTEIN (LOW PRESSURE CHROMATOGRAPY).
- ESTIMATION OF PROTEIN (SPECTROPHOTOMETER, ELISA AND PAGE).
- SAMPLE ANALYSIS IN FIBROMETER AND RESULT INTREPRATION(PT, APTT, THROMBIN AND FIBRINOGEN).
- BASIC OF MAMMALIAN CELL CULTURE (THAWING, SUBCULTURING AND CRYOPRESERVATION).
- SEEDING OF CELLS AND DRUG TESTING
- CELL VIABILITY ASSAYS (TRYPAN BLUE, MTT AND FLOWCYTOMETRY.
- CYTOSTAINING (KARYOTYPE AND IMMUNOCYTOCHEMISTRY).
- EXTRACTION OF RNA, m-RNA PURIFICATION AND ESTIMATION.
- CDNA SYNTHESIS AND AMPLIFICATION IN REAL TIME PCR AND RESULT INTERPRETATION.
- ANALYSIS OF CELLS IN FLOWCYTOMETER (CELL CYCLE AND APOPTOSIS).
* Terms and Conditions Apply