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  In Vitro Cell-based Services Pipeline

In Vitro Cell-based Services Pipeline

We provide a wide range of customized in vitro assays in various cell types including primary cells, immortalized cell lines, stem cells, cancer stem cells, 2D and/or 3D cell cultures to meet the specifications and needs of our clients. The in vitro assays include -

  • Cytotoxicity and cell proliferation assays (MTT, SRB, LDH etc.)
  • Toxicity assays for hepatocytes, hematopoietic cells, neuronal cells, endothelial cells, smooth muscle cells, fibroblasts, cardiomyocytes, monocytes, T-cells and PBMCs
  • Cellular uptake efficiency assay - by fluorescent microscopy and flow cytometry
  • Cell Death Assays- Apoptosis/Necrosis
    • Annexin-V/PI assay - by flow cytometry
    • Caspase-3/7 assay - by fluorescent microscopy, flow cytometry and RT-qPCR
    • Detection of mitochondrial membrane potential - by fluorescent microscopy and flow cytometry
    • DNA fragmentation assays: TUNEL assay (by fluorescent microscopy and flow cytometry) and Comet assays
    • Quantification of protein markers involved in apoptosis (BAX,Bcl-2, p53, γH2AX, P-AKT, etc.) - by fluorescent microscopy, flow cytometry and RT-qPCR
  • Cell Cycle Analysis
    • Using DNA-binding dyes such as PI, 7-AAD, Hoechst 33342 and 33258, DAPI - by flow cytometry
    • Analysis of cyclin dependent kinases – by fluorescent microscopy and flow cytometry
  • Functional Cell Stress Assays - by fluorescent microscopy, flow cytometry and RT-qPCR
    • ROS formation assay
    • Lipid peroxidation assay
    • Glutathione depletion assay
    • Mitochondrial membrane potential assays
    • Hypoxia signaling marker analysis
    • Anti-oxidant assays: Copper/zinc-superoxide dismutase (SOD1), manganese-SOD (SOD2), catalase (CAT), glutathione (GSH), glutathione peroxidase (GPX) and GSH reductase (GSR)
  • Cell specific marker analysis - by fluorescent microscopy (immunocytochemistry and immunohistochemistry), flow cytometry and RT-qPCR
  • Exclusive Flow Cytometry Services
    • Analysis of CD34+ cells and other stem cell markers (Ex: CD13, CD29, CD44, CD73, CD93, CD105, CD146 etc.)
    • Analysis of cell surface and intracellular markers in umbilical cord blood samples/PBMCs
    • Cell surface marker analysis for cell characterization, and/or for diagnosis and monitoring of haematological malignancies, cancer, infectious diseases and genetic disorders.
    • Measurement of G Protein-coupled Receptors (GPCRs) expression for drug discovery
    • Measurement of intracellular calcium ions
    • Measurement of ligand-receptor interactions
    • Detection of circulating endothelial cells and endothelial progenitor cells
  • Metabolism Assays: Anti-diabetic assays (glucose uptake assay, α-amylase inhibition assay, α-glucosidase inhibition assay, sucrase inhibition assay), lipids and phospholipids accumulation, LDL uptake etc.
  • Inflammation Assays: iNOS activation, NF-κB translocation, COX genes activation, interleukins etc. - by fluorescent microscopy, flow cytometry and RT-qPCR
  • Cardiac biomarkers detection for myocardial infarction (BNP, CK-MB, troponin, h-FABP, myoglobin) – by fluorescent microscopy, flow cytometry, RT-qPCR and ELISA
  • In vitro angiogenesis assay
  • Cell migration/invasion assays
  • ADME assay
  • Wound healing assay
  • Foam cell formation assay
  • Mammalian cell transfection – using calcium phosphate and lipid-mediated methods
  • Detection of transfected cells/high expression clones – by fluorescent microscopy, flow cytometry and RT-qPCR
  • Mycoplasma detection – by fluorescent microscopy (Hoechst 33342 and DAPI staining), PCR and RT-qPCR
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