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  Molecular Biology Services

Molecular Biology Services

We offer molecular services for isolates of bacterial, mammalian, insect and plant cells and our service portfolio includes –

  • Isolation of DNA, RNA and protein
  • Qualitative/quantitative analysis of DNA,RNA– by spectroscopy and agarose gel electrophoresis
  • Protein analysis
    • SDS-PAGE
    • Western blot
    • Bradford’s assay
    • Bicinchoninic acid (BCA) assay
  • DNA fragmentation studies
  • Restriction endonuclease digestion and fragment mapping
  • PCR primer design
  • Vector construction
  • DNA transformation – Preparation and transformation of competent cells
  • Molecular cloning
    • Amplification of DNA by PCR and cloning into vector of choice
    • Amplification of cDNA by RT-qPCR and cloning into vector of choice
  • Genotyping services – Analysis of genetic markers by PCR and/or RT-qPCR as applicable
    • Single-nucleotide polymorphism (SNP)
    • Microsatellites
    • Copy number variation (CNV)
    • Variable region genes
    • Homologous genes
    • Novel genes
    • 16S/18S ribosomal DNA
    • AFLP, RAPD, Cleaved Amplified Polymorphic Sequences (CAPS)/PCR-RFLP, Genotyping by sequencing (GBS)
  • RT-qPCR based gene expression profiling
    • mRNA extraction from different sample types (cultured cells, whole blood, tissues)
    • Quantification of the extracted mRNA
  • miRNA expression analysis – by RT-qPCR
  • Protein expression and purification services (from bacterial, insect and mammalian cells) – by affinity chromatography
  • Mutation analysis– by PCR, RT-qPCR and MLPA (Multiplex ligation-dependent probe amplification)
  • Antibody purification services
    • Purification methods – Ammonium sulphate precipitation and chromatography (affinity, size-exclusion and ion-exchange chromatography)
    • Quality analysis– by SDS-PAGE, western blot and ELISA
  • Mycoplasma detection–by PCR and RT-qPCR
  • Detection of viral contamination– by PCR and RT-qPCR
  • Blood coagulation factor analysis– by fibrometer
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