Other Techniques (3-6 days)

• ELISA (Direct and indirect)
• Western blotting/ Dot blotting
• Pull down assays (Immuno-precipitation)
• Peripheral blood mononuclear cells (PBMCs) isolation (Ficoll method) and their characterization
• Cell staining and fluorescence microscopy
• Flow cytometry
• Surface staining (Single color)
• Cytoplasmic and nuclear staining
• Multiple receptor staining and qualitative analysis
• Gene expression (cDNA synthesis and RT-qPCR)